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[(wa)-13(s)-352.5(m)-.6(on)-13(i-)]TJ
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[(tored)-447.5(o).8(n)-448.9(a)-445.8(96-w)10.2(ell)-447.4(p)2.3(late)-449.1(for)-447.9(4)-1.4(0)-164(m)1(in)-448.9(\(25)]TJ
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()Tj
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[(C\))-448.9(after)]TJ
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(M)Tj
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[(\)o)334.9(r2)170.2(m)]TJ
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T*
[(In)17.4(s)13.6(t)0(r)13.6(u)12.4(me)19.3(n)12.4(t)0(s)19.2(,)-261(Wi)17.4(n)12.4(o)0(o)19.1(s)0(k)15.9(i)0(,)-247(V)0(T)18.3(\))0(.)]TJ
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[(Mic)-7.6(e)]TJ
/F4 1 Tf
10.4607 0 0 10.4607 53.8015 151.9369 Tm
[(Female)-357(C57BL/6)-356.3(\()]TJ
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(H2)Tj
7.8454 0 0 6.9729 157.1527 156.5858 Tm
(b)Tj
/F4 1 Tf
10.4607 0 0 10.4607 161.5181 151.9369 Tm
[(\))-355.3(and)-359.4(BALB/c)-359.5(\()]TJ
/F11 1 Tf
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(H2)Tj
7.8454 0 0 6.9729 249.4487 156.5858 Tm
(d)Tj
/F4 1 Tf
10.4607 0 0 10.4607 254.2676 151.9369 Tm
[(\))-360.7(mice)]TJ
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[(were)-408.8(obtained)-412.7(from)-408.6(the)-403.4(a).9(nimal)-407.1(f)4.1(acility)-405.8(o)3.1(f)-413.2(t)1.9(he)-408.8(Uni-)]TJ
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[(versity)-313.7(o)3(f)-315.8(L)-4.5(euven)-316.6(\()-1.4(Leuven,)-319(B)4.2(elgium\).)-313.6(Female)]TJ
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(CD1d)Tj
/F4 1 Tf
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[(knockout)-492(\(KO\))-495.2(mice)-496.2(\(C.)-5.3(129S2-)]TJ
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(Cd1)Tj
7.8454 0 0 6.9729 208.3464 119.2252 Tm
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[(tm1G)-6.7(ru)]TJ
/F4 1 Tf
10.4607 0 0 10.4607 235.559 114.5763 Tm
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[(/J\))-494.2(on)-489.6(the)]TJ
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[(BALB/c)-413.6(backg)-5.3(round)-413.3(w)1.7(ere)-409.4(p)-1.4(urchased)-413.3(from)-414.6(Jackson)]TJ
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T*
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0 -1.1869 TD
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0 -1.1923 TD
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[(we)-3.5(re)-339.6(approv)-5.9(ed)-343.4(by)-341.9(t)-4.7(h)2.1(e)-345(a)-.3(n)-3.4(i)3.6(m)-3.3(a)-.3(l)-343.3(e)1.9(t)-4.7(h)2.1(ics)-343.6(c)-2.3(om)-3.3(mit)-4.7(t).7(ee)-345(an)-3.4(d)]TJ
T*
-.0195 Tc
[(perfor)4.8(med)-227.9(i)-.2(n)-229.3(a)1.4(ccordance)-224.1(with)-223.9(European)-223.9(regulations)4.9(.)]TJ
/F12 1 Tf
9.9626 0 0 9.9626 307.8424 712.6864 Tm
0 Tc
[(In)-334.2(vivo)]TJ
/F1 1 Tf
3.568 0 TD
(studies)Tj
/F4 1 Tf
10.4607 0 0 10.4607 319.8046 700.2707 Tm
[(Mi)17.9(ce)-304.9(\(6)-307.3(t)11(o)-312.9(8)-315.1(w)11.4(ee)19.1(ks)-303.9(ol)15.3(d\))-303.6(re)14.8(ce)20.3(iv)18.4(ed)-304.6(fo)14.6(u)12.4(r)-317.2(fo)20.1(ot)17.9(pa)18.4(d)-316.8(i)19.3(n)17.8(-)]TJ
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/F10 1 Tf
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(l)Tj
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(g/)Tj
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[(mouse\))-319(containing)-322.4(eithe)5.2(r)-324.2(the)-320(t)-1.4(hioredox)-322.4(motif)-319(o)-.2(r)-318.8(t)-1.4(he)]TJ
0 -1.1869 TD
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[(alanine)-319.8(l)1.6(oss-o)5.4(f)1(-function)-314.2(substitute)-314.4(in)-319.6(aluminum)-314.2(hy-)]TJ
0 -1.1923 TD
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[(dro)4.7(x)-3.1(ide)-434.3(a)2.5(djuvant)-430.1(\().3(alum;)-431.1(L)-2.8(ife)-428.9(T)-2.8(echnologies,)-431.1(G)-1.6(hent,)]TJ
T*
-.0182 Tc
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0 -1.1869 TD
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0 -1.1923 TD
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[(intravenously)-447.3(1)2.7(0)]TJ
7.8454 0 0 6.9729 388.0062 617.7258 Tm
0 Tc
(9)Tj
10.4607 0 0 10.4607 396.7936 613.077 Tm
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[(plaque)5.5(-forming)-441.3(units)-443(\(PFU\))-443.3(p)1.6(er)]TJ
-8.5034 -1.1923 TD
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[(mouse)-276.5(o)-.1(f)-275.5(e)-.1(ither)-275.3(A)1.1(d5-GFP)-278.6(or)-280.8(Ad5-n)5.5(u)0(ll)-274.9(vecto)5.3(r)-280.8(\(Vector)]TJ
0 -1.1869 TD
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[(BioL)-3.9(ab)-4(s,)-280.5(Ma)-4(lv)-4.2(er)-6.1(n,)-280.5(PA\))-282.7(v)-4.2(ia)-280.4(the)-278.2(t)-3(ail)-282(v)-4.2(ei)-5.6(n,)-280.5(and)-282.1(a)1.4(d)-5.7(m)-1.6(in-)]TJ
0 -1.1923 TD
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[(istration)-231.9(w).1(a)4.2(s)-236.2(re)6.4(pe)6.4(ate)6.4(d)-236(after)-230.9(1)-1.2(0)-234.3(d)2.5(ay)4(s)-236.1(w).1(hen)-231.9(i)2.6(ndicat)5.2(ed.)]TJ
T*
-.0181 Tc
[(Blood)-199.4(samples)-194.2(w)-1.3(ere)-195.6(c).8(ollecte)5(d)-199.4(10)-192.3(or)-199.8(14)-197.7(days)-199.6(after)-194.4(e)-.5(ach)]TJ
0 -1.1869 TD
[(administration)-222.5(of)-221.6(Ad5)-224.8(v)2.6(ecto)]TJ
12.1833 0 TD
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[(r)-228.4(a)1.3(s)-222.8(s)-.6(p)-5.8(e)-1.9(cied.)-226.4(To)-229.6(anal)-5.7(yze)]TJ
-12.1833 -1.1923 TD
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[(the)-275.9(p)2(ropo)5.9(rtio)5.9(ns)-280(of)-274.9(natural)-279.7(k)1.3(ille)5.9(r)-280.2(and)-279.8(C)1.7(D8)]TJ
/F5 1 Tf
7.8454 0 0 6.9729 500.4849 543.0046 Tm
0 Tc
(+)Tj
/F4 1 Tf
10.4607 0 0 10.4607 507.5715 538.3558 Tm
-.019 Tc
[(T)-279.7(c)-.2(ells)-281.8(in)]TJ
-19.0933 -1.1923 TD
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[(the)-255.1(liver,)-257.3(m)-.1(ice)-255.1(w)-1.2(ere)-255.1(k).5(i)-4.1(lled)-258.9(1)2.9(4)-257.3(d)1.2(ays)-259.1(a)2.9(fter)-253.9(the)-255.1(l)1.3(ast)-256.3(A).9(d)-4.2(5)]TJ
0 -1.1869 TD
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[(ad)-5.4(ministra)-3.7(ti)-5.3(on.)]TJ
/F1 1 Tf
9.9626 0 0 9.9626 307.8424 489.033 Tm
0 Tc
[(Hum)-8.6(oral)-336.1(respo)-9.5(nse)]TJ
/F4 1 Tf
10.4607 0 0 10.4607 319.8046 476.5605 Tm
[(To)-394.8(qu)22.5(an)22.5(ti)19.6(ta)15.7(t)11(e)-383.4(Ad)21.8(5-)17.9(s)13.6(p)0(e)20.6(c)0(i)16.5()12.4(c)-387.6(a)0(n)22.5(t)11(ib)18.5(o)12.2(d)0(y)-377.4(p)0(r)16.4(o)12.2(du)20.8(ct)19.1(io)20.7(n)12.4(,)]TJ
-1.1435 -1.1869 TD
[(96)20.1(-)13.2(w)0(e)18.2(l)0(l)-568.3(p)13.8(la)18.5(t)11(e)12.2(s)-577.1(\()0(G)19.2(r)0(e)20.3(i)0(n)20.9(e)12.2(r)-577.3(B)0(i)16.5(o)12.2(-O)19.2(n)12.4(e)12.2(,)-580.7(V)13.5(il)17(vo)22.1(o)12.2(r)0(d)16.4(e)12.2(,)-575.3(B)0(e)20.3(l)0(-)]TJ
0 -1.1923 TD
[(gi)18.4(u)12.4(m)0(\))-575.9(w)11.4(e)12.2(r)0(e)-575.9(c)0(o)20.3(a)0(t)21.1(e)0(d)-575.6(w)11.4(it)19.6(h)-583.7(A)0(d)16.4(5)-580.7(ve)16.7(c)13.4(t)11(or)-575.9(at)-575.1(1.)20.1(6)]TJ
/F5 1 Tf
20.936 0 TD
()Tj
/F4 1 Tf
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(10)Tj
7.8454 0 0 6.9729 545.6125 456.3211 Tm
(7)Tj
10.4607 0 0 10.4607 307.8424 439.1999 Tm
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[(PFU)-10.2(/)9.7(m)2.2(l)-527.4(i)-10.2(n)-520.4(1)]TJ
/F11 1 Tf
6.5903 0 TD
0 Tc
(M)Tj
/F4 1 Tf
1.4633 0 TD
[(Na)19.9(HC)22.7(O)]TJ
7.8454 0 0 6.9729 430.1857 437.4991 Tm
(3)Tj
10.4607 0 0 10.4607 434.4377 439.1999 Tm
[(/N)19.9(a)]TJ
7.8454 0 0 6.9729 451.3322 437.4991 Tm
(2)Tj
10.4607 0 0 10.4607 455.5275 439.1999 Tm
(CO)Tj
7.8454 0 0 6.9729 471.0046 437.4991 Tm
(3)Tj
10.4607 0 0 10.4607 475.1999 439.1999 Tm
[(,)-521.1(p)13.8(H)-516.5(9)0(.)20.1(5)0(,)-511.1(o)12.2(ve)22.1(r-)]TJ
-15.9987 -1.1869 TD
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[(ni)-12.4(g)6(h)-12.4(t)-346.6(a)-12.4(t)-343.6(4)]TJ
/F5 1 Tf
4.5796 0 TD
0 Tc
()Tj
/F4 1 Tf
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[(C.)-334.2(Af)21.3(te)17.9(r)-344.3(w)11.4(as)23.6(hi)15.5(n)12.4(g)0(,)-332.4(a)-342.2()56.2()0(b)17.5(l)13.9(oc)20.2(ki)16.2(n)12.4(g)0()66.1()-344.8(s)13.6(t)11(ep)-337.1(w)11.4(a)0(s)]TJ
-4.9644 -1.1923 TD
[(in)20.9(cl)16.5(u)12.4(d)0(e)20.6(d)-501.1(w)11.4(i)0(t)19.6(h)-497(2%)-490.2(bo)22.3(vi)18.4(n)12.4(e)-502.6(s)13.6(e)0(r)20.2(u)0(m)-490(a)0(l)18.5(b)0(u)22.5(m)0(i)20.9(n)-502.4(\()13.2(B)0(S)20.6(A)0(\))15.9()]TJ
T*
[(P)10.2(B)0(S)-385.8(f)13.2(or)-386.2(1)-158(h)12.4(r,)-388.4(f)13.2(o)0(l)20.7(l)0(o)15.3(w)11.4(e)12.2(d)-398.1(b)15.5(y)-396.6(di)16.9(l)13.9(u)0(t)18.1(i)0(o)20.7(n)0(s)-385.9(o)12.2(f)-398.6(s)13.6(er)20.3(um)-387(sa)23.6(m-)]TJ
0 -1.1869 TD
[(pl)16.9(e)12.2(s)-382(in)-369.3(P)10.2(B)0(S)-375(a)0(n)22.5(d)-381.9(ca)18.1(li)22.4(br)18.1(at)21.1(io)20.7(n)-383.2(s)0(a)18.2(m)12.4(pl)22.3(es)-375.2(f)13.2(o)0(r)-375.4(a)-380.2(fu)20.2(rt)19.1(h)12.4(e)0(r)]TJ
0 -1.1923 TD
[(in)20.9(cu)20.4(ba)20.1(t)11(i)0(o)20.7(n)-491.6(of)-484(9)15.5(0)-158(m)12.4(i)0(n)-483.1(a)0(t)-477.5(r)0(o)20.3(o)0(m)-479.3(t)0(e)17.9(m)12.4(pe)20.6(ra)18.1(t)11.1(u)0(r)20.4(e)0(.)-481.8(A)0(f)21.3(t)11(er)]TJ
T*
[(w)11.4(a)0(s)18.2(h)12.4(in)20.9(g,)-359.5(pl)16.9(at)21.1(e)12.2(s)-371.2(w)11.4(e)0(r)20.2(e)-372.6(p)13.8(r)0(o)20.3(b)0(e)16.9(d)-365.6(by)-359.5(ad)18.4(di)16.9(t)11(i)0(o)20.7(n)-367(of)-359.3(ho)19.2(rs)16.2(e)12.2(-)]TJ
0 -1.1869 TD
[(ra)18.1(d)13.8(i)0(s)16.7(h)-740.9(pe)15.2(r)13.4(o)0(x)16.7(i)13.9(da)18.4(se)-732.9(\(H)22.5(RP)18.3(\)-)21.1(co)20.3(nj)16.2(u)12.4(g)0(a)19.9(t)11(ed)-732.7(an)17(t)11(i)0(-)21.7(m)0(o)19.3(u)12.4(se)]TJ
0 -1.1923 TD
[(t)11(o)0(t)17.9(a)0(l)-415(I)0(g)20.3(G)-422.2(\(B)15.9(i)13.9(o)0(-)20.1(R)0(a)18.1(d)0(,)-415.2(B)0(r)21.5(u)0(s)20.6(s)0(e)20.4(l)0(s)16.7(,)-423.5(Be)20.3(lg)18.4(iu)20.9(m)12.4(\))0(,)-415.7(a)0(n)17(t)11(i-)21.7(Ig)20.2(G1)]TJ
0 -1.1869 TD
[(\(B)21.3(D)-638.9(B)0(i)16.5(o)12.2(sc)21.6(ie)20.7(nc)20.4(es)20.4(,)-640.3(E)0(r)16.1(e)12.2(m)12.4(b)0(o)22.3(d)0(e)15.2(g)0(e)22.1(m)12.4(,)-640.3(B)0(e)20.3(l)0(g)18.4(i)13.9(um)19.4(\),)-632.5(a)15.5(n)0(t)18.1(i)0(-)]TJ
0 -1.1923 TD
[(Ig)20.3(G)11.4(2)0(a)-267.2(\()0(B)15.9(D)-275.8(Bi)22(os)20.4(ci)16.5(e)12.2(n)0(c)20.4(e)0(s)20.4(\))0(,)-269.4(o)12.2(r)-279.2(a)0(n)17(t)11(i-)21.7(Ig)20.3(G)11.4(2)0(b)-272.6(\()13.2(S)12.6(a)0(n)17(t)11(a)-277.2(C)0(r)16(u)12.4(z)]TJ
T*
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[(to)-540.4(prevent)-543.6(nonspecic)-542.1(binding.)-543.1(To)-546.6(quantify)-541(the)]TJ
0 -1.1923 TD
[(proportion)-380.9(of)-381(natural)-382.7(killer)-380.1(T)-385.4(\(NKT\))-375.5(and)-381.1(CD8)]TJ
/F5 1 Tf
7.8454 0 0 6.9729 288.8503 193.9464 Tm
(+)Tj
/F4 1 Tf
10.4607 0 0 10.4607 297.1275 189.2976 Tm
(T)Tj
-22.4751 -1.1923 TD
[(cells)-420.6(from)-420.1(the)-424.9(liver,)-419.8(staining)-423(included)-419.7(anti-CD8)]TJ
0 -1.1869 TD
[(FITC)-665.7(\(eBioscience\),)-665.8(anti-CD3PE)-661.9(\(eBioscience\),)]TJ
0 -1.1923 TD
[(anti-CD19PerCP)-1044.1(\(eBioscience\),)-1045.1(anti-NKG2D)]TJ
0 -1.1869 TD
[(APC)-651.1(\(eBioscience\),)-649.5(and)-652(anti-CD4APC-H7)-643(\(BD)]TJ
0 -1.1923 TD
[(Biosciences\).)-260.4(To)-264.8(quantify)-259.2(the)-262.4(proportion)-261.6(of)-261.8(effector)]TJ
T*
[(memory)-474.7(CD4)]TJ
/F5 1 Tf
7.8454 0 0 6.9729 127.7858 119.2252 Tm
(+)Tj
/F4 1 Tf
10.4607 0 0 10.4607 137.0834 114.5763 Tm
[(T)-477.5(cells,)-475.7(surface)-480.6(staining)-477.2(included)]TJ
-7.1756 -1.1869 TD
[(anti-CD44V450)-561.5(\(BD)-563.5(Biosciences\),)-569.3(anti-CD62L)]TJ
0 -1.1923 TD
[(PE)-485.8(\(eBioscience\),)-486.9(anti-CD8PerCP)-479.7(\(eBioscience\),)]TJ
T*
[(anti-CD3APC)-339.5(\(eBioscience\),)-340.6(and)-337.7(anti-CD4APC-)]TJ
0 -1.1869 TD
[(H7)-490.2(\(BD)-493(Biosciences\))-487.2(antibodies.)-489.9(At)-490.4(least)-491.9(50,000)]TJ
0 -1.1923 TD
[(events)-280.8(were)-286.7(acquired)-280(and)-283.5(analyzed)-284.1(by)-283.6(ow)-283.7(cytom-)]TJ
T*
[(etry)-278.6(\()-84.3(MACSQuant;)-279.3(Miltenyi)-277.2(Biotec\).)]TJ
/F1 1 Tf
9.9626 0 0 9.9626 328.0251 712.6864 Tm
[(Deriva)-10.1(tion)-336.7(of)-333.4(cy)-6.4(tolytic)-336.6(CD4)]TJ
/F5 1 Tf
7.4718 0 0 6.641 456.5479 717.1085 Tm
(+)Tj
/F1 1 Tf
9.9626 0 0 9.9626 463.9747 712.6864 Tm
[(T)-336.3(cell)-337.8(clones)]TJ
/F4 1 Tf
10.4607 0 0 10.4607 339.9873 700.2707 Tm
-.0149 Tc
[(To)-409.1(induce)-409.1(cytolyti)4.4(c)-407.9(C)-1.5(D4)]TJ
/F5 1 Tf
7.8454 0 0 6.9729 455.7542 704.9196 Tm
0 Tc
(+)Tj
/F4 1 Tf
10.4607 0 0 10.4607 464.2015 700.2707 Tm
.3909 Tc
[(Tc)404.3(e)403.1(l)410.2(l)404.8(s)404.5(,C)404.4(5)406.4(7)406.4(B)404.4(L)406.5(/)406.3(6m)403.3(i)410.2(c)404.3(e)]TJ
-13.0179 -1.1923 TD
-.0149 Tc
[(were)-154.4(immunized)-158.3(f)-1.7(our)-158.6(t)1.6(imes)-158.5(\(on)-159.6(d)-1.1(ays)-158.5(1).6(,)-162.1(15,)-162(21)6(,)-162.1(a).6(nd)-158.3(28\))]TJ
T*
[(with)-408.8(ccH)5.3(exBL/6)-405.8(peptide)-403.6(o)-2.6(r)-407.8(aaHexBL/6)-405.8(peptide)-403.6(i)-.9(n-)]TJ
0 -1.1869 TD
-.0155 Tc
[(traperito)-3.3(n)2.3(e)-3.3(a)0(lly.)-401.1(T)-5.3(w)1.3(o)-398.9(w)-4.1(eeks)-403(a)0(fter)-403.1(th)-3.1(e)-398.9(l)-1.6(ast)-400.1(i)-1.6(m)-3.1(m)2.4(u)-3.1(n)2.3(i-)]TJ
0 -1.1923 TD
-.0158 Tc
[(zation)-3.4(,)-293(m)-3.4(ice)-296.2(w)1(ere)-296.2(k)-2.7(ille)-3.6(d)-294.7(an)-3.4(d)-294.7(s)-2.2(plee)-3.6(ns)-300.3(were)-296.2(rem)-3.4(o)1.8(ve)-3.6(d.)]TJ
T*
-.0153 Tc
(CD19)Tj
/F5 1 Tf
7.8454 0 0 6.9729 354.6141 642.614 Tm
0 Tc
(+)Tj
/F4 1 Tf
10.4607 0 0 10.4607 364.8188 637.9652 Tm
-.0167 Tc
[(and)-577.4(C)-3.2(D8)]TJ
/F5 1 Tf
7.8454 0 0 6.9729 409.4928 642.614 Tm
0 Tc
(+)Tj
/F4 1 Tf
10.4607 0 0 10.4607 419.6408 637.9652 Tm
-.0134 Tc
[(cel)5.9(l).5(s)-574.3(w)3.4(er)5.5(e)-575.6(t)3.1(he)4.3(n)-570(d).4(epl)5.9(e)-1.2(te)4.3(d)-574.1(f)-.2(ro)4.3(m)]TJ
-8.7581 -1.1869 TD
-.0158 Tc
[(sple)-3.6(no)-3.6(cytes)-246.1(a)-.3(nd)-240.5(t)-4.8(h)2(e)-242(r)-2.4(est)-243.2(o)1.9(f)-241(t)-4.8(he)-242(cells)-240.7(we)-3.6(re)-236.6(stim)-3.4(ulate)-3.6(d)]TJ
0 -1.1923 TD
-.0151 Tc
[(with)-409.1(mitomycin)-409.1(C)-1.7(-pretreated,)-406.1(peptide-loaded)-407.8(DCs.)]TJ
T*
-.014 Tc
[(To)-180.6(expan)3.8(d)-184.5(spe)3.7(c)-.6(i)3.8(c)-184.8(c)-.6(e)3.7(l)-.1(l)-184.3(p)5.2(opu)3.8(l)-.1(atio)3.7(ns)-184.7(be)3.7(fo)3.7(re)-180.6(ph)3.8(en)3.8(otypic)]TJ
0 -1.1869 TD
[(asse)3.7(ss)5(me)3.7(nt,)-513.4(t)2.5(hre)3.7(e)-516.6(c)4.9(o)-1.8(n)3.8(s)-.4(e)3.7(c)-.6(utive)-511.2(s)-.4(timu)3.8(latio)3.7(n)-1.6(s)-515.3(a)1.5(t)-512.4(a)1.5(n)]TJ
0 -1.1923 TD
-.0157 Tc
[(interval)-251.1(of)-251.8(10)-249.5(days)-251.4(were)-252.8(performed.)]TJ
/F1 1 Tf
9.9626 0 0 9.9626 328.0251 550.8282 Tm
0 Tc
[(APC)-340.8(cell)-337.8(death)-338.8(assay)]TJ
/F4 1 Tf
10.4607 0 0 10.4607 339.9873 538.3558 Tm
[(DCs)-340.9(were)-346.3(rst)-339.7(stained)-346.5(with)-341.4(CytoRed)-342(according)]TJ
-1.1435 -1.1923 TD
[(to)-589.1(the)-582.1(manufacturers)-585.6(instructions.)-589.8(Peptide)-584.8(aa-)]TJ
0 -1.1869 TD
[(HexBL/6)-391(\(1)]TJ
/F10 1 Tf
5.5334 0 TD
(l)Tj
/F11 1 Tf
.5528 0 TD
(M)Tj
/F4 1 Tf
.9484 0 TD
[(\))-393.2(was)-387.7(added)-397(to)-388.6(10)]TJ
7.8454 0 0 6.9729 489.9967 518.1164 Tm
(4)Tj
10.4607 0 0 10.4607 498.444 513.4676 Tm
[(DCs)-389.7(before)-392.6(co-)]TJ
-16.2914 -1.1923 TD
[(culturing)-483.2(overnight)-477.8(with)-476.9(2)]TJ
/F5 1 Tf
12.9908 0 TD
()Tj
/F4 1 Tf
.7154 0 TD
(10)Tj
7.8454 0 0 6.9729 482.9668 505.644 Tm
(4)Tj
10.4607 0 0 10.4607 492.3211 500.9951 Tm
[(specic)-476.2(CD4)]TJ
/F5 1 Tf
7.8454 0 0 6.9729 553.8329 505.644 Tm
(+)Tj
/F4 1 Tf
10.4607 0 0 10.4607 563.1305 500.9951 Tm
(T)Tj
-22.4751 -1.1923 TD
[(cells)-182.2(generated)-179.9(with)-178.8(either)-179.4(ccHexBL/6)-179.8(or)-180.3(aaHexBL/)]TJ
0 -1.1869 TD
[(6)-250.1(epitope.)-247.6(DCs)-248.8(only,)-245(or)-250.7(DCs)-248.8(cocultured)-245.5(with)-249.3(T)-249.9(cells)]TJ
0 -1.1923 TD
[(in)-407.2(the)-403.3(absence)-403.7(of)-408.1(peptide,)-403.2(were)-405.9(used)-403.2(as)-409.9(controls.)]TJ
T*
[(After)-402.7(18)-169.6(hr,)-403.1(the)-403.3(cells)-404.4(were)-405.9(stained)-400.7(with)-406.4(annexin-)]TJ
0 -1.1869 TD
[(VAPC)-562.6(for)-562.7(15)-164.2(min)-562.8(at)-558.8(room)-562.1(temperature)-559.3(in)-564.4(the)]TJ
0 -1.1923 TD
[(dark.)-541.2(An)-537.8(additional)-541.5(volume)-533.8(of)-543.6(annexin)-534.5(binding)]TJ
T*
[(buffer)-304.7(\(100)]TJ
/F10 1 Tf
5.2408 0 TD
(l)Tj
/F4 1 Tf
.5528 0 TD
[(l/well;)-304.7(BD)-305.7(Biosciences\))-308.3(was)-306.4(added)-304.9(be-)]TJ
-5.7936 -1.1869 TD
[(fore)-295.7(centrifugation.)-289.7(The)-290.3(cells)-296(were)-292.1(resuspended)-290(in)]TJ
0 -1.1923 TD
[(0.2%)-318.4(BSAPBS)-320.6(\(100)]TJ
/F10 1 Tf
9.6307 0 TD
(l)Tj
/F4 1 Tf
.5528 0 TD
[(l/well\))-323.2(and)-316(analyzed)-322(by)-321.5(ow)]TJ
-10.1835 -1.1923 TD
(cytometry.)Tj
/F1 1 Tf
9.9626 0 0 9.9626 328.0251 351.5527 Tm
[(Immu)-10.7(nohistoc)-9.3(hemis)-8.1(try)]TJ
/F4 1 Tf
10.4607 0 0 10.4607 339.9873 339.0802 Tm
[(Mi)17.9(ce)-364.5(w)11.4(e)0(r)14.8(e)-372.6(ki)16.2(ll)17(ed)-364.2(an)17.1(d)-371(l)0(i)17(v)0(e)16.7(r)0(s)-363.2(w)11.4(er)14.8(e)-372.6(p)0(e)20.6(r)0(f)15.8(u)0(s)15.2(e)0(d)-364.2(w)11.4(it)14.1(h)]TJ
-1.1435 -1.1869 TD
[(P)10.2(B)0(S)-142(v)0(i)18.4(a)-147.1(th)12.6(e)-144.9(i)0(n)15.5(f)0(e)20.1(r)0(i)11.1(o)12.2(r)-149.2(v)0(e)16.7(n)0(a)-140.1(c)0(a)18.1(v)0(a)-137.2(b)0(e)16.9(f)0(o)14.6(r)0(e)-136.9(b)0(e)16.9(i)0(n)15.5(g)-147.3(re)14.8(mo)19.3(ve)16.7(d.)]TJ
0 -1.1923 TD
[(Af)15.9(te)17.9(r)-360.5()0(x)16.9(a)0(t)15.7(i)0(o)15.3(n)-356.1(w)11.4(i)0(t)14.1(h)-356.1(fo)14.6(rm)20.5(al)13.1(de)15.2(hy)16.9(de)-347.9(\(4)17.9(%\))17(,)-358.5(8)0(-)]TJ
/F10 1 Tf
19.077 0 TD
(l)Tj
/F4 1 Tf
.5474 0 TD
-.0138 Tc
[(m-)-13.8(th)-13.8(ic)-13.8(k)]TJ
-19.6244 -1.1923 TD
0 Tc
[(cr)16(yo)16.7(se)15(ct)13.6(io)20.7(ns)-272.1(we)12.8(re)-267(pr)11(e)12.2(p)0(a)13(r)0(e)20.3(d)0(.)-274.3(G)11.4(re)14.8(en)-273.4(u)19.4(or)14.8(es)15(ce)14.8(n)12.4(t)-281.6(pr)16.4(o-)]TJ
0 -1.1869 TD
[(te)17.9(in)-401.8(\(G)13.8(F)10.2(P)10.2(\))-409.5(ex)16.7(pr)16.4(es)15(si)16.7(on)-403.5(w)11.4(a)0(s)-399.1(t)0(h)12.6(e)12.2(n)-410.3(e)0(v)16.7(a)0(l)18.5(u)0(a)17.1(t)0(e)12.5(d)-409(by)-397.4(im)15.5(-)]TJ
0 -1.1923 TD
[(mu)14(n)12.4(o)0(h)13.8(i)0(s)16.7(t)0(o)17.9(c)0(h)15(e)0(m)13.8(i)0(s)16.7(t)11(ry)12.5(.)-434.4(T)0(o)-427.4(q)0(u)17(a)0(n)11.6(t)11(it)14.1(at)15.7(e)-432.2(G)0(F)16.2(P)]TJ
/F5 1 Tf
7.8454 0 0 6.9729 526.1667 281.4802 Tm
(+)Tj
/F4 1 Tf
10.4607 0 0 10.4607 535.0676 276.8314 Tm
[(ce)14.8(ll)17(s)-436.2(i)0(n)]TJ
-19.7924 -1.1923 TD
[(th)18.1(e)-546(l)0(i)17(v)0(e)16.7(r)0(,)-534.8(c)0(r)16(y)0(o)16.7(s)0(e)15(c)0(t)19.1(i)0(o)15.3(n)0(s)-537.6(w)11.4(er)14.8(e)-540.6(i)0(n)15.5(c)0(u)15(b)0(a)20.1(t)0(e)12.4(d)-539(ov)16.7(er)14.8(ni)15.5(gh)16.9(t)]TJ
0 -1.1869 TD
[(wi)14.5(t)11(h)-301.9(ra)18.1(bb)14.7(it)-289.4(an)11.6(t)11(i)0(-)16.3(G)0(F)16.2(P)-298.7(\(d)10.8(il)17(ut)18.1(ed)-293.7(1:)20.1(40)14.7(0\))-291.1(an)17(d)-300.6(t)0(h)12.6(e)12.2(n)-301.9(p)0(r)16.4(o)0(-)]TJ
0 -1.1923 TD
[(be)16.9(d)-333.1(f)0(o)20.1(r)-333.4(45)-147.9(mi)15.5(n)-329(w)0(i)14.5(t)0(h)-323.4(g)0(o)16.7(a)0(t)-320.3(a)0(n)17(t)0(i)14.1(-)0(r)15.8(a)0(b)14.7(b)0(i)18.6(t)-330.4(Ig)14.8(G)11.4()0(H)13.8(R)0(P)-323.2(\()0(d)16.2(i)0(-)]TJ
T*
[(lu)15.5(te)17.9(d)-316.8(1)0(:)14.6(3)0(0)20.1(0)0(\))-307.3(\()0(b)12.4(o)12.2(th)-312.5(fr)15.8(o)12.2(m)-318.2(L)10.2(i)0(f)16.3(e)-318.4(T)10.2(e)0(c)14.8(h)0(n)14(o)12.2(lo)15.3(gi)13(e)12.2(s)0(\))16(.)-315.2(Si)15.7(gn)16.9(al)13.1(s)]TJ
0 -1.1869 TD
[(we)18.2(re)-532.5(de)20.6(ve)16.7(lo)15.3(pe)15.2(d)-539(b)0(y)-527.5(a)0(d)18.4(d)0(i)11.4(t)11(io)15.3(n)-535(o)0(f)-527.3(d)0(i)11.4(a)0(m)17.1(i)0(n)15.5(o)12.2(be)16.9(nz)14.5(id)16.9(in)15.5(e)]TJ
0 -1.1923 TD
[(\(S)15(ig)18.4(ma)17.1(-A)15.9(ld)16.9(ri)11.1(ch)15(\).)-188.1(T)10.2(o)-199.1(qu)17(an)17(ti)14.1(t)11(a)0(t)15.7(e)-199.1(K)11.4(u)0(p)15.4(f)0(f)15.6(e)0(r)-191.1(c)0(e)20.3(l)0(l)11.6(s)-197.8(in)-185(li)11.6(ve)16.7(r)]TJ
T*
[(se)15(ct)19.1(io)15.3(ns)15.2(,)-662(c)0(r)10.6(y)0(o)16.7(s)0(e)20.4(c)0(t)13.6(i)0(o)15.3(n)0(s)-656.9(w)11.4(er)14.8(e)-665.2(i)0(n)15.5(c)0(u)20.4(b)0(a)14.7(t)0(e)17.9(d)-663.7(ov)16.7(er)14.8(ni)15.5(gh)16.9(t)]TJ
0 -1.1869 TD
[(wi)14.5(t)11(h)-204.4(ra)18.1(t)-200.3(a)0(n)17(t)0(i)14.1(-)0(m)14.9(o)12.2(us)15.2(e)-199.1(F)0(4)14.9(/)0(8)14.6(0)-195.9(\(A)15.9(bD)-195.3(S)12.6(e)0(r)14.8(o)0(t)17.9(e)0(c)14.8(,)-201.3(P)10.2(u)0(c)15(h)0(h)14(e)0(i)15.3(m)12.4(,)]TJ
0 -1.1923 TD
[(Ge)18.2(rm)15(an)17(y\))-220.8(at)-222.8(a)-223(1)0(:)14.6(1)0(0)14.7(0)-223(di)11.5(lu)15.5(t)11(i)0(o)15.3(n)-226(an)11.6(d)-224.7(t)0(h)12.6(e)12.2(n)-231.5(s)0(t)19.2(a)0(i)13.1(n)12.4(ed)-223.3(w)11.4(i)0(t)14.1(h)]TJ
T*
[(ra)18.1(bb)14.7(it)-175.6(an)17(t)11(i)0(-)16.3(r)0(a)12.6(t)-178.6(Ig)14.8(G)16(HR)17.3(P)-179.5(\()0(D)13.8(a)0(k)17.7(o)0(,)-172.9(H)0(e)16(v)0(e)16.7(r)0(l)16.5(e)0(e)13.6(,)-179.7(Be)14.9(lg)18.4(iu)15.5(m\))]TJ
0 -1.1869 TD
[(at)-260.7(a)-266.4(1)0(:)20.1(3)0(0)14.7(0)-266.4(di)16.9(lu)15.5(t)11(i)0(o)15.3(n)0(.)-259.4(A)0(f)15.9(t)0(e)17.9(r)-268.4(wa)16(sh)15.2(in)15.5(g,)-256.5(si)16.7(gn)16.9(al)13.1(s)-262.8(w)0(e)12.8(r)0(e)-256.2(d)0(e)15.2(-)]TJ
0 -1.1923 TD
[(te)17.9(ct)13.6(ed)-207(wi)14.5(t)11(h)-215.2(Cy)12.5(3)-212.2(t)0(y)15.5(r)0(a)18.1(m)0(i)15.5(d)0(e)-207(\()0(P)12.6(e)12.2(rk)15.7(in)15.5(El)16.5(me)13.9(r,)-209.6(Z)12.4(a)0(v)14.5(e)0(n)13.8(t)11(em)19.3(,)]TJ
0 -1.1869 TD
[(Be)14.9(lg)18.4(iu)15.5(m\))14.9(.)-255.5(G)11.4(FP)]TJ
/F5 1 Tf
7.8454 0 0 6.9729 398.8912 119.622 Tm
(+)Tj
/F4 1 Tf
10.4607 0 0 10.4607 405.8645 114.9732 Tm
[(ce)14.8(ll)17(s)-257.4(a)0(n)17(d)-251.8(Ku)18.4(pf)10.8(fe)20.1(r)-257.6(c)0(e)20.3(l)0(l)11.6(s)-252(we)18.2(re)-245.3(id)11.4(e)12.2(n)0(t)12.6(i)0(-)]TJ
-7.4411 -1.1923 TD
[(e)13.8(d)-365.6(a)0(n)17(d)-365.6(en)13.8(u)12.4(m)0(e)13.9(r)0(a)18.1(t)0(e)17.9(d)-371(by)-359.4(m)12.4(i)0(c)16.5(r)0(o)14.8(s)0(c)16.2(o)0(p)15.2(y)-369.5(\(A)15.9(xi)18.4(o)-367.1(I)0(m)17.4(a)0(g)14.5(e)12.2(r;)]TJ
T*
[(Ca)18.1(rl)-238.2(Ze)19.2(is)16.7(s,)-236.5(G)11.4(o)]TJ
5.9291 .0217 TD
()Tj
.4065 -.0217 TD
[(tt)16.7(in)15.5(ge)16.7(n,)-232.3(Ge)12.8(rm)20.5(an)11.6(y\))17.7(.)]TJ
/F1 1 Tf
9.9626 0 0 9.9626 328.0251 64.8 Tm
[(Sta)-7.4(tistical)-340.3(analysi)-8.3(s)]TJ
/F4 1 Tf
10.4607 0 0 10.4607 339.9873 52.3275 Tm
[(Data)-884.2(are)-885.6(expressed)-887.8(as)-886.9(means)]TJ
/F5 1 Tf
16.0475 0 TD
()Tj
/F4 1 Tf
.7154 0 TD
[(SEM.)-886.7(The)]TJ
-17.9064 -1.1923 TD
(MannWhitney)Tj
/F11 1 Tf
7.4736 0 TD
(U)Tj
/F4 1 Tf
1.0622 0 TD
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